Diatom DNA metabarcoding – Initiation training – 7 to 10 November 2022

Du 7 au 10 novembre, 13 personnes se sont retrouvées pour le "Diatom DNA metabarcoding - Initiation training" à l'UMR Carrtel, Thonon.

Program

Monday 7 November

Morning: arrival

Afternoon:

13:30: Roundtable presentation, presentation of the objective of the training (Cécile, Alexis)

14:00: General presentation of metabarcoding steps (Alexis)

14:30: General presentation of the workflow for diatoms (Agnès, Frédéric)

Presentations of the protocols de labo extraction, PCR (Cécile)

Reference barcoding data (Frédéric, Alexis)

Tuesday 8 November

9:00: Extractions and PCR (Cécile, Alexis)

13:00: Lunch

14:30: Bioinformatic pipeline: step by step mentoring (Alexis)

Wednesday 9 November

9:00:        - In lab: Gel, revealing PCR (Cécile)

                -  Theory: the libraries construction, sequencing principles, length of amplicons + primers + tags …(Cécile, Alexis)

12:00: Lunch

14:00: Bioinformatic pipeline: do it alone (Alexis)

Thursday 10 November

9:00: Presentation of ring tests results, preservation test (Cécile, Agnès)

10:00: biomonitoring indices, comparison to microscopy (Frédéric, Agnès)

11:00: Skills quiz and debriefing

Training participants

Cécile Chardon, Louis Jacas, Alexis Canino, Frédéric Rimet, Agnès Bouchez